Panorama of DNA hairpin folding observed via diffusion-decelerated fluorescence correlation spectroscopy.
نویسندگان
چکیده
Based on a confocal microscopy platform, we extended the FCS time window by three orders of magnitude to the s timescale by attaching a polystyrene microsphere. We simultaneously monitored the relaxations of multiple intermediates involved in DNA hairpin folding, thus offering a much more detailed view of the kinetics of hairpin folding experimentally.
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ورودعنوان ژورنال:
- Chemical communications
دوره 48 59 شماره
صفحات -
تاریخ انتشار 2012